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custom built nikon ti microscope  (Nikon)


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    Structured Review

    Nikon custom built nikon ti microscope
    Custom Built Nikon Ti Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59676 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom+built+nikon+ti+microscope/Objectives/us12590961-297-10-11
    Average 99 stars, based on 59676 article reviews
    custom built nikon ti microscope - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Single-particle Tracking:

    Article Title: FOXA1 mutations co-opt nascent transcription factor networks in partnership with androgen receptor to enhance prostate tumorigenicity
    Article Snippet: .. SPT experiments were conducted on a custom-built Nikon TI microscope with a 100x/NA 1.49 oil-immersion objective to allow for Highly-inclined Laminated optical (HiLo) illumination ( ). .. About 18-24 hours prior to imaging, LnCAP-AR cells were plated on MatTek #1.5 glass-bottom dishes by first coating the dishes in 0.01% poly-L-lysine and rinsing with distilled water.

    Article Title: FOXA1 mutations co-opt nascent transcription factor networks in partnership with androgen receptor to enhance prostate tumorigenicity.
    Article Snippet: .. Single particle tracking at fast framerates (fast SPT) SPT experiments were conducted on a custom-built Nikon TI microscope with a 100x/NA 1.49 oil-immersion objective to allow for Highly-inclined Laminated optical (HiLo) illumination. .. 39 About 18–24 h prior to imaging, LnCAP-AR cells were plated on MatTek #1.5 glass-bottom dishes by first coating the dishes in 0.01% poly-L-lysine and rinsing with distilled water.

    Article Title: RNA polymerase II depletion from the inactive X chromosome territory is not mediated by physical compartmentalization.
    Article Snippet: .. Two-dimensional single particle tracking by photo-activated localization microscopy (2D SPT-PALM) was performed as previously described26,27 on a custom-built Nikon TI microscope (Nikon Instruments Inc.) equipped with a ×100/NA 1.49 oil-immersion TIRF objective (Nikon apochromat CFI Apo TIRF ×100 Oil), EM-CCD camera (Andor, iXon Ultra 897; frame-transfer mode; vertical shift speed 0.9 μs; −70 °C), a perfect focusing system to correct for axial drift and motorized laser illumination (Ti-TIRF, Nikon). ..

    Article Title: FOXA1 mutations co-opt nascent transcription factor networks in partnership with androgen receptor to enhance prostate tumorigenicity
    Article Snippet: .. SPT experiments were conducted on a custom-built Nikon TI microscope with a 100x/NA 1.49 oil-immersion objective to allow for Highly-inclined Laminated optical (HiLo) illumination. .. About 18–24 h prior to imaging, LnCAP-AR cells were plated on MatTek #1.5 glass-bottom dishes by first coating the dishes in 0.01% poly-L-lysine and rinsing with distilled water.

    Microscopy:

    Article Title: FOXA1 mutations co-opt nascent transcription factor networks in partnership with androgen receptor to enhance prostate tumorigenicity
    Article Snippet: .. SPT experiments were conducted on a custom-built Nikon TI microscope with a 100x/NA 1.49 oil-immersion objective to allow for Highly-inclined Laminated optical (HiLo) illumination ( ). .. About 18-24 hours prior to imaging, LnCAP-AR cells were plated on MatTek #1.5 glass-bottom dishes by first coating the dishes in 0.01% poly-L-lysine and rinsing with distilled water.

    Article Title: FOXA1 mutations co-opt nascent transcription factor networks in partnership with androgen receptor to enhance prostate tumorigenicity.
    Article Snippet: .. Single particle tracking at fast framerates (fast SPT) SPT experiments were conducted on a custom-built Nikon TI microscope with a 100x/NA 1.49 oil-immersion objective to allow for Highly-inclined Laminated optical (HiLo) illumination. .. 39 About 18–24 h prior to imaging, LnCAP-AR cells were plated on MatTek #1.5 glass-bottom dishes by first coating the dishes in 0.01% poly-L-lysine and rinsing with distilled water.

    Article Title: Recovering mixtures of fast-diffusing states from short single-particle trajectories
    Article Snippet: .. sptPALM experiments were performed with a custom-built Nikon TI microscope equipped with a ×100/NA 1.49 oil-immersion TIRF objective (Nikon apochromat CFI Apo TIRF 100X Oil), an EMCCD camera (Andor iXon Ultra 897), a perfect focus system to account for axial drift, an incubation chamber maintaining a humidified 37°C atmosphere with 5% CO 2 , and a laser launch with 405 nm (140 mW, OBIS, Coherent), 488 nm, 561 nm, and 633 nm (all 1 W, Genesis Coherent) laser lines. ..

    Article Title: Enhancer–promoter interactions and transcription are largely maintained upon acute loss of CTCF, cohesin, WAPL or YY1
    Article Snippet: .. In brief, the experiments were performed with a custom-built Nikon TI microscope equipped with a 100X/NA 1.49 oil-immersion TIRF objective (Nikon apochromat CFI Apo TIRF 100X Oil), an EMCCD camera (Andor iXon Ultra897), a perfect focus system to correct for axial drift and motorized laser illumination (Ti-TIRF, Nikon), and an incubation chamber maintaining a humidified 37 °C atmosphere with 5% CO2 for the sample and the objective. .. Excitation was achieved using the following laser lines: 561 nm (1 W, Genesis Coherent) for JF549/PA-JF549 and TMR dyes; 633 nm (1 W, Genesis Coherent) for JF646/PA-JF646 dyes; 405 nm (140 mW, OBIS, Coherent) for all photo-activation experiments.

    Article Title: RNA polymerase II depletion from the inactive X chromosome territory is not mediated by physical compartmentalization.
    Article Snippet: .. Two-dimensional single particle tracking by photo-activated localization microscopy (2D SPT-PALM) was performed as previously described26,27 on a custom-built Nikon TI microscope (Nikon Instruments Inc.) equipped with a ×100/NA 1.49 oil-immersion TIRF objective (Nikon apochromat CFI Apo TIRF ×100 Oil), EM-CCD camera (Andor, iXon Ultra 897; frame-transfer mode; vertical shift speed 0.9 μs; −70 °C), a perfect focusing system to correct for axial drift and motorized laser illumination (Ti-TIRF, Nikon). ..

    Article Title: FOXA1 mutations co-opt nascent transcription factor networks in partnership with androgen receptor to enhance prostate tumorigenicity
    Article Snippet: .. SPT experiments were conducted on a custom-built Nikon TI microscope with a 100x/NA 1.49 oil-immersion objective to allow for Highly-inclined Laminated optical (HiLo) illumination. .. About 18–24 h prior to imaging, LnCAP-AR cells were plated on MatTek #1.5 glass-bottom dishes by first coating the dishes in 0.01% poly-L-lysine and rinsing with distilled water.

    Article Title: Recovering mixtures of fast-diffusing states from short single-particle trajectories
    Article Snippet: .. After the final incubation, cells were changed into phenol red- free medium for imaging. sptPALM sptPALM experiments were performed with a custom- built Nikon TI microscope equipped with a ×100/ NA 1.49 oil- immersion TIRF objective (Nikon apochromat CFI Apo TIRF 100X Oil), an EMCCD camera (Andor iXon Ultra 897), a perfect focus system to account for axial drift, an incubation chamber maintaining a humidified 37°C atmosphere with 5% CO2, and a laser launch with 405 nm (140 mW, OBIS, Coherent), 488 nm, 561 nm, and 633 nm (all 1 W, Genesis Coherent) laser lines. ..

    Article Title: Quantum dot conjugated virus spike protein for cell-based bio-sensing systems and drug screening
    Article Snippet: .. Single-Molecule Fluorescence Microscopy Single-molecule imaging experiments were conducted on a custom-built Nikon Ti microscope coupled with a 100× Oil-immersion objective lens (N.A.=1.49), a multi-band dichroic (405/488/561/633 BrightLine quad-band bandpass filter, Semrock, USA) and a piezo z-stage (ASI, USA). ..

    Incubation:

    Article Title: Recovering mixtures of fast-diffusing states from short single-particle trajectories
    Article Snippet: .. sptPALM experiments were performed with a custom-built Nikon TI microscope equipped with a ×100/NA 1.49 oil-immersion TIRF objective (Nikon apochromat CFI Apo TIRF 100X Oil), an EMCCD camera (Andor iXon Ultra 897), a perfect focus system to account for axial drift, an incubation chamber maintaining a humidified 37°C atmosphere with 5% CO 2 , and a laser launch with 405 nm (140 mW, OBIS, Coherent), 488 nm, 561 nm, and 633 nm (all 1 W, Genesis Coherent) laser lines. ..

    Article Title: Enhancer–promoter interactions and transcription are largely maintained upon acute loss of CTCF, cohesin, WAPL or YY1
    Article Snippet: .. In brief, the experiments were performed with a custom-built Nikon TI microscope equipped with a 100X/NA 1.49 oil-immersion TIRF objective (Nikon apochromat CFI Apo TIRF 100X Oil), an EMCCD camera (Andor iXon Ultra897), a perfect focus system to correct for axial drift and motorized laser illumination (Ti-TIRF, Nikon), and an incubation chamber maintaining a humidified 37 °C atmosphere with 5% CO2 for the sample and the objective. .. Excitation was achieved using the following laser lines: 561 nm (1 W, Genesis Coherent) for JF549/PA-JF549 and TMR dyes; 633 nm (1 W, Genesis Coherent) for JF646/PA-JF646 dyes; 405 nm (140 mW, OBIS, Coherent) for all photo-activation experiments.

    Article Title: Recovering mixtures of fast-diffusing states from short single-particle trajectories
    Article Snippet: .. After the final incubation, cells were changed into phenol red- free medium for imaging. sptPALM sptPALM experiments were performed with a custom- built Nikon TI microscope equipped with a ×100/ NA 1.49 oil- immersion TIRF objective (Nikon apochromat CFI Apo TIRF 100X Oil), an EMCCD camera (Andor iXon Ultra 897), a perfect focus system to account for axial drift, an incubation chamber maintaining a humidified 37°C atmosphere with 5% CO2, and a laser launch with 405 nm (140 mW, OBIS, Coherent), 488 nm, 561 nm, and 633 nm (all 1 W, Genesis Coherent) laser lines. ..

    Imaging:

    Article Title: Recovering mixtures of fast-diffusing states from short single-particle trajectories
    Article Snippet: .. After the final incubation, cells were changed into phenol red- free medium for imaging. sptPALM sptPALM experiments were performed with a custom- built Nikon TI microscope equipped with a ×100/ NA 1.49 oil- immersion TIRF objective (Nikon apochromat CFI Apo TIRF 100X Oil), an EMCCD camera (Andor iXon Ultra 897), a perfect focus system to account for axial drift, an incubation chamber maintaining a humidified 37°C atmosphere with 5% CO2, and a laser launch with 405 nm (140 mW, OBIS, Coherent), 488 nm, 561 nm, and 633 nm (all 1 W, Genesis Coherent) laser lines. ..

    Article Title: Quantum dot conjugated virus spike protein for cell-based bio-sensing systems and drug screening
    Article Snippet: .. Single-Molecule Fluorescence Microscopy Single-molecule imaging experiments were conducted on a custom-built Nikon Ti microscope coupled with a 100× Oil-immersion objective lens (N.A.=1.49), a multi-band dichroic (405/488/561/633 BrightLine quad-band bandpass filter, Semrock, USA) and a piezo z-stage (ASI, USA). ..

    Fluorescence:

    Article Title: Quantum dot conjugated virus spike protein for cell-based bio-sensing systems and drug screening
    Article Snippet: .. Single-Molecule Fluorescence Microscopy Single-molecule imaging experiments were conducted on a custom-built Nikon Ti microscope coupled with a 100× Oil-immersion objective lens (N.A.=1.49), a multi-band dichroic (405/488/561/633 BrightLine quad-band bandpass filter, Semrock, USA) and a piezo z-stage (ASI, USA). ..



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